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Downregulation of Pax3 expression correlates with acquired GFAP expression during NSC differentiation towards astrocytes
Author(s) -
Liu Yan,
Zhu Hui,
Liu Mei,
Du Jinfeng,
Qian Yuyan,
Wang Yongjun,
Ding Fei,
Gu Xiaosong
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.02.034
Subject(s) - glial fibrillary acidic protein , astrocyte , gfap stain , pax3 , transcription factor , microbiology and biotechnology , downregulation and upregulation , biology , nestin , intermediate filament , cellular differentiation , neural stem cell , chemistry , central nervous system , immunohistochemistry , stem cell , immunology , cell , gene , neuroscience , biochemistry , cytoskeleton
Glial fibrillary acidic protein (GFAP) is a principal intermediate filament in mature astrocytes of the central nervous system (CNS), and the regulation of GFAP transcription has not been well understood yet. In the present study, we reported paired box 3 protein (Pax3) as a novel regulator of GFAP transcription, which could bind the promoter region of GFAP and down regulate the GFAP level during the serum‐induced astrocyte differentiation of neural stem cells (NSCs). Moreover, the overexpression and suppression of Pax3 could inhibit and promote NSC differentiation, respectively. These data suggest that Pax3 negatively regulates GFAP expression during astrocyte differentiation in vitro.