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Cytotoxicity of red fluorescent protein DsRed is associated with the suppression of Bcl‐xL translation
Author(s) -
Zhou Jun,
Lin Jian,
Zhou Cuihong,
Deng Xiaoyan,
Xia Bin
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.02.013
Subject(s) - cytotoxicity , hela , green fluorescent protein , microbiology and biotechnology , chemistry , bcl xl , biology , apoptosis , biochemistry , cell , programmed cell death , in vitro , gene
Red fluorescent protein (RFP) DsRed and its variants are widely applied in live‐cell imaging experiments. However, a major factor that restricts the application of DsRed is its cytotoxicity. Here, we report that DsRed and its variant DsRed‐Express2 inhibit the expression of B‐cell lymphoma‐extra large (Bcl‐xL) in HeLa cells by translational regulation. Over‐expression of Bcl‐xL can reduce the cytotoxicity of DsRed. Meanwhile, Turbo RFP, a mutant RFP from Entacmaea quadricolor , does not affect Bcl‐xL expression, suggesting that cytotoxic mechanisms of RFP from different species may be varied. Our results reveal a possible mechanism for the cytotoxicity of DsRed, providing a potential strategy to improve the application of DsRed and its variants.