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The DYW‐E‐PPR protein MEF14 is required for RNA editing at site matR ‐1895 in mitochondria of Arabidopsis thaliana
Author(s) -
Verbitskiy Daniil,
Härtel Barbara,
Zehrmann Anja,
Brennicke Axel,
Takenaka Mizuki
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.01.037
Subject(s) - rna editing , mutant , arabidopsis thaliana , biology , gene , open reading frame , genetics , rna , mitochondrial dna , arabidopsis , mitochondrion , peptide sequence
We here identify the PPR protein MEF14 of the DYW subclass as a specific trans ‐factor required for C to U editing of site matR ‐1895 by genetic mapping of an EMS induced editing mutant in Arabidopsis thaliana . The wild type Col MEF14 gene complements mutant protoplasts. A T‐DNA insertion in the MEF14 gene abolishes detectable editing at the matR ‐1895 site. Lack of RNA editing at the matR ‐1895 site does not alter the level of mature and precursor nad1 mRNA molecules. Such RNA editing mutants can be used to analyse the function of genes like this maturase related reading frame in plant mitochondria.