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Synechocystis sp. PCC 6803 mutant lacking both photosystems exhibits strong carotenoid‐induced quenching of phycobilisome fluorescence
Author(s) -
Rakhimberdieva Marina G.,
Kuzminov Fedor I.,
Elanskaya Irina V.,
Karapetyan Navassard V.
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2011.01.013
Subject(s) - phycobilisome , allophycocyanin , photosystem , quenching (fluorescence) , fluorescence , photochemistry , chemistry , biophysics , synechocystis , mutant , photosystem ii , biology , biochemistry , cyanobacteria , photosynthesis , phycocyanin , genetics , physics , optics , bacteria , gene
Blue light induced quenching in a Synechocystis sp. PCC 6803 strain lacking both photosystems is only related to allophycocyanin fluorescence. A fivefold decrease in the fluorescence level in two bands near 660 and 680 nm is attributed to different allophycocyanin forms in the phycobilisome core. Some low‐heat sensitive component inactivated at 53 °C is involved in the quenching process. Enormous allophycocyanin fluorescence in the absence of the photosystems reveals a dark stage in this quenching. Thus, we present evidence that light activation of the carotenoid‐binding protein and formation of a quenching center within the phycobilisome core in vivo are discrete events in a multistep process.