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Cloning and functional characterization of TCRP1, a novel gene mediating resistance to cisplatin in an oral squamous cell carcinoma cell line
Author(s) -
Gu Yixue,
Fan Shasha,
Xiong Yan,
Peng Bo,
Zheng Guopei,
Yu Yanhui,
Ouyang Yongmei,
He Zhimin
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.12.045
Subject(s) - cisplatin , gene , biology , gene expression , microbiology and biotechnology , microarray analysis techniques , microarray , cell culture , cancer research , drug resistance , apoptosis , genetics , chemotherapy
To explore the mechanisms of chemotherapy resistance, we previously established a multi‐drug resistant cell line, Tca8113/Pingyangmycin (Tca8113/PYM) and identified differential expression in known genes and ESTs using microarray analysis. From among those ESTs we have now identified a novel gene producing an mRNA of 1834 nucleotides translated into a protein having 235 amino acids. This gene was denominated as tongue cancer resistance‐associated protein 1 gene ( TCRP1 , accession number: EF363480 ). We further determined its functional characteristics. The results demonstrate that TCRP1 mediates a specific resistance to cisplatin in Tca8113 cells by reducing the cisplatin‐induced apoptosis. This suggests that TCRP1 might be a novel molecular target to develop agents to reverse cisplatin‐induced chemoresistance.