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Expression of S100A8/A9 in HaCaT keratinocytes alters the rate of cell proliferation and differentiation
Author(s) -
Voss Andreas,
Bode Günther,
Sopalla Claudia,
Benedyk Malgorzata,
Varga Georg,
Böhm Markus,
Nacken Wolfgang,
Kerkhoff Claus
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.12.037
Subject(s) - hacat , involucrin , microbiology and biotechnology , keratinocyte , s100a8 , cellular differentiation , filaggrin , cell growth , cell culture , chemistry , biology , inflammation , immunology , biochemistry , gene , genetics , atopic dermatitis
S100A8/A9 promotes NADPH oxidase in HaCaT keratinocytes and subsequently increases NFκB activation, which plays important roles in the balance between epidermal growth and differentiation. S100A8/A9‐positive HaCaT cells present with a significantly reduced rate of cell division and greater expression of two keratinocyte differentiation markers, involucrin and filaggrin, than control cells. S100A8/A9 mutants fail to enhance NFκB activation, TNFα‐induced IL‐8 gene expression and NFκB p65 phosphorylation, and S100A8/A9‐positive cells demonstrate better cell survival in forced suspension culture than mutant cells. S100A8/A9 is induced in epithelial cells in response to stress. Therefore, S100A8/A9‐mediated growth arrest could have implications for tissue remodeling and repair.