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Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release
Author(s) -
Gil Carles,
Falqués Anton,
Sarró Eduard,
Cubí Roger,
Blasi Juan,
Aguilera José,
Itarte Emilio
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.12.029
Subject(s) - lipid raft , phosphorylation , syntaxin , neurotransmitter , chemistry , synaptosome , biochemistry , protein kinase a , glutamate receptor , raft , kinase , microbiology and biotechnology , protein phosphorylation , biology , membrane protein , membrane , receptor , organic chemistry , copolymer , polymer
In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent‐resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin‐1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho‐specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin‐1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol‐enriched microdomains.