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PU.1 directly regulates retinoic acid‐induced expression of RIG‐G in leukemia cells
Author(s) -
Gu Zhi-Min,
Liu Chuan-Xu,
Wu Shao-Fang,
Zhao Meng,
Xu Han-Zhang,
Liu Wei,
Zhou Hu-Chen,
Chen Guo-Qiang,
Wu Ying-Li
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.12.021
Subject(s) - retinoic acid , electrophoretic mobility shift assay , gene silencing , luciferase , microbiology and biotechnology , reporter gene , rig i , chemistry , small hairpin rna , gene expression , tretinoin , rna interference , function (biology) , biology , biochemistry , gene , transfection , gene knockdown , rna
RIG‐G is a retinoic acid‐ or interferon‐induced gene with potential anti‐proliferation function. However, the mechanism underlying ATRA‐induced RIG‐G induction is not completely understood. Here, we demonstrate that ATRA up‐regulates the expression of PU.1, which in turn directly binds to the promoter and increases the expression of RIG‐G gene. Luciferase reporter assay and electrophoretic mobility shift assay reveal that PU.1 preferentially binds to one of the two putative binding sites on the RIG‐G promoter. Moreover, silencing of PU.1 by shRNA markedly inhibited ATRA‐ but not IFNα‐induced expression of RIG‐G. These data provide new insight into the mechanism of ATRA‐induced RIG‐G expression.