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Up‐regulation of the vitamin C transporter SVCT2 upon differentiation and depolarization of myotubes
Author(s) -
Low Marcela,
Sandoval Daniel,
Morales Bryan,
Nualart Francisco,
Henríquez Juan Pablo
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.12.020
Subject(s) - myogenesis , myocyte , biology , microbiology and biotechnology , vitamin c , depolarization , intracellular , skeletal muscle , transporter , endocrinology , biochemistry , gene
In addition to its role as a strong antioxidant, vitamin C regulates the differentiation of several cell lineages. In vertebrate skeletal muscle, the vitamin C transporter SVCT2 is preferentially expressed in slow muscle fibers. To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. SVCT2 expression increases in cultures of both, slow and fast muscle‐derived myoblasts, as they fuse to form mainly fast myotubes. Interestingly, we found that SVCT2 could be positively modulated by potassium‐induced depolarization of myotubes. These findings suggest that SVCT2‐mediated uptake of vitamin C could play diverse roles on skeletal muscle development and physiology.