SIRT1 deficiency attenuates MPP + ‐induced apoptosis in dopaminergic cells | Zendy

Park Geunyeong | Zendy; Jeong Joo-Won | Zendy; Kim Ja-Eun | Zendy

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SIRT1 deficiency attenuates MPP + ‐induced apoptosis in dopaminergic cells

Author(s) -

Park Geunyeong,

Jeong Joo-Won,

Kim Ja-Eun

Publication year - 2011

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2010.11.048

Subject(s) - sirtuin 1 , apoptosis , transfection , chemistry , cytotoxicity , sh sy5y , microbiology and biotechnology , nad+ kinase , activator (genetics) , resveratrol , poly adp ribose polymerase , neuroprotection , cell culture , biology , biochemistry , in vitro , downregulation and upregulation , pharmacology , enzyme , neuroblastoma , gene , genetics , polymerase

One of the functions mediated by sirtuin 1 (SIRT1), the NAD + ‐dependent protein deacetylase, has been suggested to be neuroprotective since resveratrol, a SIRT1 activator, inhibits 1‐methyl‐4‐phenylpyridinium ion (MPP + )‐induced cytotoxicity. In this study, we show that SIRT1 siRNA transfection blocks MPP + ‐induced apoptosis in SH‐SY5Y cells. The ratio of potential pro‐apoptotic BNIP2 to antiapoptotic BCL‐xL was attenuated in SIRT1‐deficient cells following MPP + treatment. In addition, BNIP2 shRNA‐transfected cells showed reduced cleavage of PARP‐1, while BNIP2 overexpression intensified the cleavage in MPP + ‐treated SH‐SY5Y cells, suggesting that BNIP2 participates in the MPP + ‐induced apoptosis. Overall, these data imply that SIRT1 may mediate MPP + ‐induced cytotoxicity, possibly through the regulation of BNIP2.

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