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A novel zinc‐carboxypeptidase SURO‐1 regulates cuticle formation and body morphogenesis in Caenorhabditis elegans
Author(s) -
Kim Tai Hoon,
Kim Yeon Jung,
Cho Jin Won,
Shim Jaegal
Publication year - 2011
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.11.020
Subject(s) - caenorhabditis elegans , mutant , cuticle (hair) , biology , morphogenesis , carboxypeptidase , microbiology and biotechnology , enzyme , biochemistry , genetics , gene
Cuticle formation and molting are critical for the development of Caenorhabditis elegans . To understand cuticle formation more clearly, we screened for suppressors in transgenic worms that expressed dominant ROL‐6 collagen proteins. The suro‐1 mutant, which is mild dumpy, exhibited a different ROL‐6::GFP localization pattern compared to other Dpy mutants. We identified mutations in three suro‐1 mutants, and found that suro‐1 (ORF R11A5.7) encodes a putative zinc‐carboxypeptidase homologue. The expression of this enzyme in the hypodermis and the genetic interactions between this enzyme and other collagen‐modifying enzyme mutants suggest a regulatory role in collagen processing and cuticle organization for this novel carboxypeptidase. These findings aid our understanding of cuticle formation during worm development.