Premium
Identification of a cofilin‐like actin‐binding site on translationally controlled tumor protein (TCTP)
Author(s) -
Tsarova Katsiaryna,
Yarmola Elena G.,
Bubb Michael R.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.054
Subject(s) - cofilin , actin , actin remodeling , microbiology and biotechnology , chemistry , actin binding protein , biology , actin cytoskeleton , biochemistry , cytoskeleton , cell
Translationally controlled tumor protein (TCTP) expression is suppressed during cancer cell reversion to a non‐malignant phenotype. We identified a primary sequence of TCTP with homology to ADF/cofilin. We confirm that a synthetic peptide corresponding to this sequence binds specifically to actin and is displaced from actin by cofilin. TCTP peptide has higher affinity for G‐actin than F‐actin and does not block actin‐filament depolymerization by cofilin. These results suggest that TCTP may channel active cofilin to F‐actin, enhancing the cofilin‐activity cycle in invasive tumor cells. Loss of TCTP may result in sequestration of active cofilin by a monomeric pool of actin.