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An efflux pump is involved in secretion of newly synthesized siderophore by Pseudomonas aeruginosa
Author(s) -
Hannauer Mélissa,
Yeterian Emilie,
Martin Lois W.,
Lamont Iain L.,
Schalk Isabelle J.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.051
Subject(s) - siderophore , efflux , pyoverdine , periplasmic space , pseudomonas aeruginosa , secretion , microbiology and biotechnology , bacteria , chemistry , fluorescence microscope , fluorescence , biochemistry , biology , escherichia coli , genetics , physics , quantum mechanics , gene
Pseudomonas aeruginosa secretes the fluorescent siderophore, pyoverdine (PVD), to enable iron acquisition. Epifluorescence microscopy and cellular fractionation were used to investigate the role of an efflux pump, PvdRT‐OpmQ, in PVD secretion. Bacteria lacking this efflux pump accumulated PVD, or a fluorescent precursor, in the periplasm, due to their inability to efficiently secrete into the media newly synthesized PVD. PvdRT‐OpmQ is only the second system identified for secretion of newly synthesized siderophores by Gram negative bacteria.