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Activation of the NLRP3 inflammasome by intracellular poly I:C
Author(s) -
Rajan Jayant V.,
Warren Sarah E.,
Miao Edward A.,
Aderem Alan
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.036
Subject(s) - inflammasome , mda5 , tlr3 , rna silencing , cytosol , endosome , microbiology and biotechnology , aim2 , rna , intracellular , chemistry , secretion , transfection , biology , virology , rna interference , gene , biochemistry , innate immune system , enzyme , receptor , toll like receptor
Several RNA viruses can be detected by the inflammasome, which promotes IL‐1β and IL‐18 secretion, but the underlying mechanisms of detection remain unclear. Cytosolic dsRNA is a replication intermediate of many RNA viruses. We show here that transfection of the dsRNA analogue poly I:C activates the NLRP3 inflammasome via a pathway requiring endosomal acidification. This detection is independent of the other poly I:C sensors: TLR3 and MDA5. These results suggest a mechanism by which cytosolic dsRNA produced during viral infection could activate the NLRP3 inflammasome.