Premium
A free radical‐generating system regulates APP metabolism/processing
Author(s) -
Recuero María,
Muñoz Teresa,
Aldudo Jesús,
Subías Marta,
Bullido María J.,
Valdivieso Fernando
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.028
Subject(s) - oxidative stress , secretion , amyloid precursor protein , metabolism , inducer , amyloid precursor protein secretase , programmed cell death , apoptosis , chemistry , microbiology and biotechnology , lipid metabolism , biochemistry , alzheimer's disease , biology , medicine , disease , gene
Oxidative stress, a risk factor in the pathophysiology of Alzheimer's disease, is intimately associated with aging. We previously reported that the X‐XOD free radical generating system acts as a modulator of lipid metabolism and a mild inducer of apoptotic death. Using the same cell model, the present study examines the metabolism/processing of the amyloid precursor protein (APP). Prior to inducing cell death, X‐XOD promoted the secretion of α‐secretase‐cleaved soluble APP (sAPPα) and increased the level of APP carboxy‐terminal fragments produced by α and γ secretase (αCTF and γCTF/AICD). In contrast, it reduced the activity of β‐secretase and the level of secreted Aβ. The present results indicate that mild oxidative stress maintained throughout culturing regulates APP metabolism/processing in SK‐N‐MC human neuroblastoma cells.