Premium
Methyl‐beta‐cyclodextrin induces mitochondrial cholesterol depletion and alters the mitochondrial structure and bioenergetics
Author(s) -
Ziolkowski Wieslaw,
Szkatula Michał,
Nurczyk Artur,
Wakabayashi Takashi,
Kaczor Jan Jacek,
Olek Robert Antoni,
Knap Narcyz,
Antosiewicz Jedrzej,
Wieckowski Mariusz Roman,
Wozniak Michal
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.023
Subject(s) - bioenergetics , mitochondrion , lipid raft , lipid microdomain , microbiology and biotechnology , inner mitochondrial membrane , chemistry , raft , efflux , biophysics , beta (programming language) , biology , biochemistry , cholesterol , membrane , organic chemistry , copolymer , computer science , programming language , polymer
There is growing evidence of mitochondrial membrane raft‐like microdomains that are involved in the apoptotic pathway. The aim of this study was to investigate the effect of methyl‐beta‐cyclodextrin (MβCD), being a well‐known lipid microdomain disrupting agent and cholesterol chelator, on the structure and bioenergetics of rat liver mitochondria (RLM). We observed that MβCD decreases the function of RLM, induces changes in the mitochondrial configuration state and decreases the calcium chloride‐induced swelling. These data suggest that disruption of mitochondrial raft‐like microdomains by cholesterol efflux on one hand impairs mitochondrial bioenergetics, but on the other hand it protects the mitochondria from swelling.