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MALAT‐1 enhances cell motility of lung adenocarcinoma cells by influencing the expression of motility‐related genes
Author(s) -
Tano Keiko,
Mizuno Rie,
Okada Tomoko,
Rakwal Randeep,
Shibato Junko,
Masuo Yoshinori,
Ijiri Kenichi,
Akimitsu Nobuyoshi
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.008
Subject(s) - motility , gene knockdown , gene silencing , biology , gene , microbiology and biotechnology , cell , gene expression , small interfering rna , rna , cancer research , genetics
MALAT‐1, a long non‐coding RNA, is associated with metastasis, but its role in the metastatic process remains unknown. Here, we show that short‐interfering RNA‐mediated MALAT‐1 silencing impaired in vitro cell motility of lung cancer cells and influenced the expression of numerous genes. In these genes, knockdown of any one of CTHRC1 , CCT4 , HMMR , or ROD1 clearly inhibited cell migration. In MALAT‐1 knockdown cells, pre‐mRNA levels were decreased in some but not all genes. Thus, our findings suggest that MALAT‐1 is a novel class of non‐coding RNA that promotes cell motility through transcriptional and post‐transcriptional regulation of motility related gene expression.