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Δ160p53 is a novel N‐terminal p53 isoform encoded by Δ133p53 transcript
Author(s) -
Marcel Virginie,
Perrier Stéphane,
Aoubala Mustapha,
Ageorges Sylvain,
Groves Michael J.,
Diot Alexandra,
Fernandes Kenneth,
Tauro Sudhir,
Bourdon Jean-Christophe
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.10.005
Subject(s) - gene isoform , gene knockdown , microbiology and biotechnology , biology , gene , mutagenesis , messenger rna , genetics , mutation
p53 gene expresses several protein isoforms modulating p53‐mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, Δ160p53, lacking the first 159 residues. By knockdown experiments and site‐directed mutagenesis, we show that Δ160p53 is encoded by Δ133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of Δ160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and Δ133p53 protein expression but not the one of Δ160p53. Overall, these results show that the Δ133p53 transcript generates two different p53 isoforms, Δ133p53 and Δ160p53.