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Sequence discrimination of the Zα domain of human ADAR1 during B–Z transition of DNA duplexes
Author(s) -
Seo Yeo-Jin,
Ahn Hee-Chul,
Lee Eun-Hae,
Bang Jongchul,
Kang Young-Min,
Kim Hee-Eun,
Lee Yeon-Mi,
Kim Kyungmin,
Choi Byong-Seok,
Lee Joon-Hwa
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.09.036
Subject(s) - dna , z dna , transition (genetics) , duplex (building) , sequence (biology) , microbiology and biotechnology , stereochemistry , chemistry , biology , biophysics , biochemistry , gene
The Zα domain of human ADAR1 (Zα ADAR1 ) preferentially binds Z‐DNA rather than B‐DNA with high binding affinity. Zα ADAR1 binds to the Z‐conformation of both non‐CG‐repeat DNA duplexes and a d(CGCGCG) 2 duplex similarly. We performed NMR experiments on complexes between the Zα ADAR1 and non‐CG‐repeat DNA duplexes, d(CACGTG) 2 or d(CGTACG) 2 , with a variety of protein‐DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG) 2 in the previous study suggests that Zα ADAR1 exhibits the sequence preference of d(CGCGCG) 2 ≫ d(CACGTG) 2 > d(CGTACG) 2 through multiple sequence discrimination steps during the B–Z transition.