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Role of prostaglandin E2 in peptidoglycan mediated iNOS expression in mouse peritoneal macrophages in vitro
Author(s) -
Dahiya Yogesh,
Pandey Rajeev Kumar,
Bhatt Kunal H.,
Sodhi Ajit
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.09.009
Subject(s) - autocrine signalling , paracrine signalling , peptidoglycan , microbiology and biotechnology , nitric oxide synthase , extracellular , prostaglandin e2 , nitric oxide , biology , gene expression , endogeny , in vitro , prostaglandin e , chemistry , biochemistry , enzyme , endocrinology , gene , receptor
Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/autocrine signalling induced by endogenous biomolecules that are synthesised as a result of host cell–microbe interaction. In this paper we demonstrate that iNOS expression in mouse peritoneal macrophages in response to bacterial peptidoglycan (PGN) is a secondary effect requiring autocrine signalling of endogenously produced prostaglandin E2, and that PGN stimulation is mandatory, but not sufficient in itself, for induction of iNOS expression.