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Long range dynamic effects of point‐mutations trap a response regulator in an active conformation
Author(s) -
Bobay Benjamin G.,
Thompson Richele J.,
Hoch James A.,
Cavanagh John
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.08.051
Subject(s) - point mutation , regulator , response regulator , mutation , conformational change , biophysics , loop (graph theory) , biology , active site , protein structure , genetics , microbiology and biotechnology , bacterial protein , biochemistry , gene , enzyme , mathematics , combinatorics
When a point‐mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point‐mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This ‘conformational trapping’ results in functionally hyperactive Spo0F. Consequently, point‐mutations are seen to affect functionally critical motions both close to and far from the mutational site.