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Epitope tag‐induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase
Author(s) -
Maradeo Marie E.,
Skibbens Robert V.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.08.020
Subject(s) - cohesin , protein subunit , microbiology and biotechnology , chemistry , immunoprecipitation , acetyltransferase , biology , biochemistry , acetylation , meiosis , gene
Ctf7/Eco1‐dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag‐induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf7/eco1 mutant cells, suggesting that Smc1 and Smc3 head domains are in direct contact with each other and also with Mcd1/Scc1. Thus, cohesin complexes may be much more compact than commonly portrayed. We further demonstrate that mutation in ELG1 or RFC5 anti‐establishment genes suppress tag‐induced lethality, consistent with the notion that the replication fork regulates Ctf7/Eco1.