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Induction of endosomal/lysosomal pathways in differentiating osteoblasts as revealed by combined proteomic and transcriptomic analyses
Author(s) -
Taniguchi Takako,
Kido Shinsuke,
Yamauchi Emiko,
Abe Masahiro,
Matsumoto Toshio,
Taniguchi Hisaaki
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.07.055
Subject(s) - endosome , transcriptome , proteome , microbiology and biotechnology , osteoblast , extracellular matrix , lysosome , chemistry , quantitative proteomics , messenger rna , proteomics , biology , in vitro , gene expression , biochemistry , gene , intracellular , enzyme
We have analyzed proteome changes associated with bone‐forming osteoblast differentiation by quantitative differential proteomic and transcriptomic analyses using in vitro differentiation model. Sixty nine proteins were found up‐regulated (>2‐fold) and 18 were down‐regulated (<0.5‐fold) at protein level. The mRNA levels of these proteins were then analyzed by quantitative real‐time PCR combined with clustering analysis. The most prominent cluster with increased protein and mRNA levels contains endosomal and lysosomal proteins, demonstrating the drastic induction of degradative endosomal/lysosomal pathways in osteoblasts. Osteoblasts, therefore, are involved not only in the synthesis but also in the turnover of the extracellular matrix proteins such as collagens.