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Oxazolone‐induced over‐expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model
Author(s) -
Ma Yanju,
Semba Shingo,
Maemoto Atsuo,
Takeuchi Masayuki,
Kameshita Isamu,
Ishida Atsuhiko,
Kato Sanae,
Katoh Tsuyoshi,
Liu Yunpeng,
Taniguchi Takanobu
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.07.054
Subject(s) - lamina propria , oxazolone , colitis , focal adhesion , ptk2 , immunohistochemistry , cancer research , tyrosine kinase , chemistry , epithelium , microbiology and biotechnology , kinase , biology , pathology , signal transduction , immunology , medicine , protein kinase a , mitogen activated protein kinase kinase
We examined the change of protein tyrosine kinases (PTKs) expression levels in colonic epithelial cells isolated from mice in which colitis was induced by oxazolone administration, using the monoclonal antibody YK34, which cross‐reacts with a wide variety of PTKs. We identified focal adhesion kinase (FAK) and found the expression level increased due to the induction of colitis. Furthermore, we found that there was a positive correlation between FAK expression and the severity of colitis. Also, FAK expression localized in the colonic epithelium but not in the lamina propria, implying FAK functions in epithelial cells during colitis formation and/or wound repairing.