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Generation of trans‐mitochondrial mito‐mice by the introduction of a pathogenic G13997A mtDNA from highly metastatic lung carcinoma cells
Author(s) -
Yokota Mutsumi,
Shitara Hiroshi,
Hashizume Osamu,
Ishikawa Kaori,
Nakada Kazuto,
Ishii Rie,
Taya Choji,
Takenaga Keizo,
Yonekawa Hiromichi,
Hayashi Jun-Ichi
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.07.048
Subject(s) - mitochondrial dna , phenotype , biology , mitochondrion , lung , microbiology and biotechnology , mitochondrial disease , cancer research , gene , genetics , medicine
To investigate the effects of respiration defects on the disease phenotypes, we generated trans‐mitochondrial mice (mito‐mice) by introducing a mutated G13997A mtDNA, which specifically induces respiratory complex I defects and metastatic potentials in mouse tumor cells. First, we obtained ES cells and chimeric mice containing the G13997A mtDNA, and then we generated mito‐mice carrying the G13997A mtDNA via its female germ line transmission. The three‐month‐old mito‐mice showed complex I defects and lactate overproduction, but showed no other phenotypes related to mitochondrial diseases or tumor formation, suggesting that aging or additional nuclear abnormalities are required for expression of other phenotypes.