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Simulated microgravity promotes nitric oxide‐supported angiogenesis via the iNOS–cGMP–PKG pathway in macrovascular endothelial cells
Author(s) -
Siamwala Jamila H.,
Majumder Syamantak,
Tamilarasan K.P.,
Muley Ajit,
Reddy Seerapu H.,
Kolluru Gopi Krishna,
Sinha Swaraj,
Chatterjee Suvro
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.06.039
Subject(s) - nitric oxide , angiogenesis , cyclic guanosine monophosphate , nitric oxide synthase , chemistry , microbiology and biotechnology , endothelium , biochemistry , biology , medicine , endocrinology , organic chemistry
Angiogenesis is a physiological process involving the growth of blood vessel in response to specific stimuli. The present study shows that limited microgravity treatments induce angiogenesis by activating macrovascular endothelial cells. Inhibition of nitric oxide production using pharmacological inhibitors and inducible nitric oxide synthase (iNOS) small interfering ribo nucleic acid (siRNA) abrogated microgravity induced nitric oxide production in macrovascular cells. The study further delineates that iNOS acts as a molecular switch for the heterogeneous effects of microgravity on macrovascular, endocardial and microvascular endothelial cells. Further dissection of nitric oxide downstream signaling confirms that simulated microgravity induces angiogenesis via the cyclic guanosine monophosphate (cGMP)–PKG dependent pathway.