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Loss of Trx‐2 enhances oxidative stress‐dependent phenotypes in Drosophila
Author(s) -
Tsuda Manabu,
Ootaka Ryousuke,
Ohkura Chiaki,
Kishita Yoshihito,
Seong Ki-Hyeon,
Matsuo Takashi,
Aigaki Toshiro
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.06.034
Subject(s) - phenotype , paraquat , oxidative stress , catalase , thioredoxin , superoxide dismutase , glutathione , mutant , drosophila (subgenus) , gene , biology , biochemistry , reactive oxygen species , hydrogen peroxide , microbiology and biotechnology , genetics , enzyme
Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss‐of‐function phenotypes of Trx‐2 in Drosophila , and found that the mutant flies are hyper‐susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx‐2 mutants express high levels of anti‐oxidant genes, such as superoxide dismutase , catalase , and glutathione synthetase . This is the first demonstration of biochemical and physiological consequences caused by loss of Trx‐2 in Drosophila .