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Weird fingers: Functional analysis of WIP domain proteins
Author(s) -
Appelhagen Ingo,
Huep Gunnar,
Lu Gui-Hua,
Strompen Georg,
Weisshaar Bernd,
Sagasser Martin
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.06.007
Subject(s) - zinc finger , mutant , tilling , green fluorescent protein , biology , function (biology) , subfamily , microbiology and biotechnology , genetics , computational biology , chemistry , gene , transcription factor
WIP proteins form a plant specific subfamily of C2H2 zinc finger (ZF) proteins. In this study, we functionally characterized the WIP domain, which consists of four ZF motifs, and discuss molecular functions for WIP proteins. Mutations in each of the ZFs lead to loss of function of the TT1/WIP1 protein in Arabiopsis thaliana . SV40 type nuclear localisation signals were detected in two of the ZFs and functionally characterized using GFP fusions as well as new mutant alleles identified by TILLING. Promoter swap experiments showed that selected WIP proteins are partially able to take over TT1 function. Activity of the AtBAN promoter, a potential TT1 target, could be increased by the addition of TT1 to the TT2–TT8–TTG1 regulatory complex.