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Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis‐mediated cell death
Author(s) -
Gupta Anurag Kumar,
Joshi Manjunath B.,
Philippova Maria,
Erne Paul,
Hasler Paul,
Hahn Sinuhe,
Resink Therese J.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.06.006
Subject(s) - neutrophil extracellular traps , microbiology and biotechnology , nadph oxidase , programmed cell death , endothelial stem cell , cell culture , extracellular , endothelium , chemistry , biology , immunology , inflammation , reactive oxygen species , in vitro , apoptosis , biochemistry , genetics , endocrinology
Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co‐culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL‐8 by activated EC. Extended neutophil/EC co‐culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.