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Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated β‐amino acid
Author(s) -
Thasaopporn,
Prapagdee Benjaphorn,
Rangkadilok Nuchanart,
Sallabhan Ratiboot,
Aye Seaim Lwin,
Ruchirawat Somsak,
Loprasert Suvit
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.06.005
Subject(s) - bacillus subtilis , lipopeptide , amino acid , bacillaceae , bacillales , chemistry , stereochemistry , antibiotics , antifungal , biochemistry , bacteria , microbiology and biotechnology , biology , genetics
Subtulene A, a new cyclic lipopeptide, was isolated from the culture broth of Bacillus subtilis SSE4. This antibiotic compound contained the seven common α‐amino acids, l ‐Asn‐1, d ‐Tyr‐2, d ‐Asn‐3, l ‐Gln‐4, l ‐Pro‐5, d ‐Asn‐6, l ‐Ser‐7 and the unique β‐amino acid‐8 present in the iturin family. 1D and 2D NMR, as well as MS analyses, identified the β‐amino acid as 3‐amino‐13‐methyltetradec‐8‐enoic acid, an Iso C15 long chain β‐amino acid. B. subtilis SSE4 was also found to produce iturin A. B. subtilis SSE4 culture filtrate exhibited both antifungal and antibacterial activities.