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Glucose and lactate regulate maitotoxin‐activated Ca 2+ entry in spermatogenic cells: The role of intracellular [Ca 2+ ]
Author(s) -
Reyes Juan G.,
Osses Nelson,
Knox Marcela,
Darszon Alberto,
Treviño Claudia L.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.05.051
Subject(s) - intracellular , chemistry , biochemistry , biophysics , biology
Maitotoxin (MTX), a potent polyether marine biotoxin, induces Ca 2+ entry in different mammalian cells by activation of Ca 2+ channels. The identity and modulation of the MTX‐activated Ca 2+ entry pathway is not known. In this work, we show, for the first time, that glucose and lactate can modulate the excitability of spermatogenic cell MTX‐activated Ca 2+ channels. Physiological and pharmacological evidences indicate that glucose and lactate differentially affect MTX‐activated Ca 2+ entry mainly through changes that these substrates induce on intracellular Ca 2+ stores and the concentration of intracellular Ca 2+ ([Ca 2+ ] i ) in spermatogenic cells. Our findings strongly suggest that MTX‐activated Ca 2+ channels in spermatogenic cells can be regulated by a Ca 2+ ‐CaM‐dependent protein kinase.