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Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6)
Author(s) -
Chien Chen-Li,
Wu Yu-Shuo,
Lai Hsing-Lin,
Chen Yen-Hui,
Jiang Si-Tse,
Shih Chia-Ming,
Lin Sui-Shan,
Chang Chen,
Chern Yijuang
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.05.004
Subject(s) - adenylyl cyclase , agonist , endocrinology , reabsorption , knockout mouse , medicine , receptor , forskolin , chemistry , stimulation , biology , kidney , biochemistry
Adenylyl cyclase (AC) type VI (AC6) is a calcium‐inhibitable enzyme which produces cAMP upon stimulation. Herein, we characterized the specific role of AC6 in the kidneys using two AC6‐knockout mouse lines. Immunohistochemical staining revealed that AC6 exists in the tubular parts of the nephron and collecting duct. Activities of AC evoked by forskolin or a selective agonist of the V2 vasopressin receptor were lower in the kidneys of AC6‐null mice compared to those of wildtype mice. Results of a metabolic cage assay and dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) showed for the first time that AC6 plays a critical role in regulating water homeostasis.