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Transforming growth factor‐β inhibits nephronectin‐induced osteoblast differentiation
Author(s) -
Fang Ling,
Kahai Shireen,
Yang Weining,
He Chengyan,
Seth Arun,
Peng Chun,
Yang Burton B.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.04.074
Subject(s) - osteoblast , transforming growth factor beta , cellular differentiation , microbiology and biotechnology , tgf beta signaling pathway , transforming growth factor , transforming growth factor, beta 3 , chemistry , beta (programming language) , transfection , cell growth , biology , endocrinology , growth factor , gene , biochemistry , in vitro , tgf alpha , receptor , computer science , programming language
We used cDNA microarray to identify transforming growth factor beta (TGF‐β) responsive target genes during osteoblast development and found that nephronectin (Npnt) is one such gene that is significantly down‐regulated. Here we report the role of TGF‐β in regulating Npnt‐mediated osteoblast differentiation. We found that the effect of TGF‐β on Npnt expression is associated with a change in cell morphology in a dose‐dependent manner. Npnt‐induced osteoblast differentiation was also inhibited by TGF‐β, which changed cell morphology from cuboidal to fibroblastic, an indication that osteoblast differentiation was disrupted. Furthermore, TGF‐β inhibited differentiation of osteoblasts transfected with various truncated Npnt constructs, suggesting that TGF‐β can exert a down‐stream effect on Npnt function. Our results suggest that TGF‐β can inhibit osteoblast differentiation through various mechanisms.