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Duality of the carbohydrate‐recognition system of Pseudomonas aeruginosa ‐II lectin (PA‐IIL)
Author(s) -
Wu Albert M.,
Gong Yu-Ping,
Li Chia-Chen,
Gilboa-Garber Nechama
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.04.026
Subject(s) - pseudomonas aeruginosa , lectin , carbohydrate , chemistry , microbiology and biotechnology , pseudomonadales , biochemistry , biology , bacteria , genetics
The study of Pseudomonas aeruginosa ‐II lectin (PA‐IIL) complexes with Man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. Here, the roles of Man oligomers and complexes in PA‐IIL carbohydrate‐recognition were studied by both enzyme‐linked lectinosorbent and inhibition assays. From the results obtained, it is proposed that high density weak –OH conformation as seen in yeast mannan is also an important PA‐IIL recognition factor. This finding provides a peculiar concept of the duality of PA‐IIL recognition system for l Fucα1→ and related complexes and for high density Manα1→ complexes present in polymannosylated target macromolecules.