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Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications
Author(s) -
Löfblom J.,
Feldwisch J.,
Tolmachev V.,
Carlsson J.,
Ståhl S.,
Frejd F.Y.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.04.014
Subject(s) - computational biology , nanotechnology , protein engineering , molecular engineering , scaffold , chemistry , computer science , biology , biochemistry , materials science , enzyme , database
Affibody molecules are a class of engineered affinity proteins with proven potential for therapeutic, diagnostic and biotechnological applications. Affibody molecules are small (6.5 kDa) single domain proteins that can be isolated for high affinity and specificity to any given protein target. Fifteen years after its discovery, the Affibody technology is gaining use in many groups as a tool for creating molecular specificity wherever a small, engineering compatible tool is warranted. Here we summarize recent results using this technology, propose an Affibody nomenclature and give an overview of different HER2‐specific Affibody molecules. Cumulative evidence suggests that the three helical scaffold domain used as basis for these molecules is highly suited to create a molecular affinity handle for vastly different applications.