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Salicylate‐induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane
Author(s) -
Kumano Shun,
Iida Koji,
Ishihara Kenji,
Murakoshi Michio,
Tsumoto Kouhei,
Ikeda Katsuhisa,
Kumagai Izumi,
Kobayashi Toshimitsu,
Wada Hiroshi
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.04.010
Subject(s) - prestin , chemistry , mutant , cytoplasm , microbiology and biotechnology , motor protein , mutation , biochemistry , gene , biology , microtubule
Prestin is a key molecule for mammalian hearing. The present study investigated changes in characteristics of prestin by culturing prestin‐transfected cells with salicylate, an antagonist of prestin. As a result, the plasma membrane localization of prestin bearing a mutation in the GTSRH sequence, which normally accumulates in the cytoplasm, was recovered. Moreover, the nonlinear capacitance of the majority of the mutants, which is a signature of prestin activity, was also recovered. Thus, the present study discovered a new effect of salicylate on prestin, namely, the promotion of the plasma membrane expression of prestin mutants in an active state.