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Single‐molecule analysis of the Escherichia coli replisome and use of clamps to bypass replication barriers
Author(s) -
Georgescu Roxana E.,
Yao Nina Y.,
O'Donnell Mike
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.04.003
Subject(s) - replisome , okazaki fragments , dna replication , dna polymerase , biology , microbiology and biotechnology , dna clamp , clamp , escherichia coli , genetics , dna , circular bacterial chromosome , computer science , eukaryotic dna replication , gene , polymerase chain reaction , reverse transcriptase , clamping , computer vision
The process of chromosome duplication faces many obstacles. One way to circumvent blocks is to hop over them by placing a new clamp on a downstream primer. This resembles lagging strand synthesis, where the tight grip of polymerase to the clamp and DNA must be overcome upon completing each Okazaki fragment so it can transfer to new primed sites. This review focuses on recent single‐molecule studies showing that Escherichia coli Pol III can hop from one clamp to another without leaving the replication fork. This capability provides a means to circumvent obstacles like transcription or DNA lesions without fork collapse.