Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in  Glycine max | Zendy

Shibuya Masaaki | Zendy; Nishimura Kazuya | Zendy; Yasuyama Nao | Zendy; Ebizuka Yutaka | Zendy

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Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max

Author(s) -

Shibuya Masaaki,

Nishimura Kazuya,

Yasuyama Nao,

Ebizuka Yutaka

Publication year - 2010

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2010.03.037

Subject(s) - triterpene , aglycone , glycosyltransferase , saponin , chemistry , biochemistry , transferase , biosynthesis , rhamnose , glycoside , galactose , stereochemistry , enzyme , medicine , alternative medicine , pathology

Triterpene saponins are a diverse group of compounds with a structure consisting of a triterpene aglycone and sugars. Identification of the sugar‐transferase involved in triterpene saponin biosynthesis is difficult due to the structural complexity of triterpene saponin. Two glycosyltransferases from Glycine max , designated as GmSGT2 and GmSGT3, were identified and characterized. In vitro analysis revealed that GmSGT2 transfers a galactosyl group from UDP‐galactose to soyasapogenol B monoglucuronide, and that GmSGT3 transfers a rhamnosyl group from UDP‐rhamnose to soyasaponin III. These results suggest that soyasaponin I is biosynthesized from soyasapogenol B by successive sugar transfer reactions.

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