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Targeted knock‐out of a gene encoding sulfite reductase in the moss Physcomitrella patens affects gametophytic and sporophytic development
Author(s) -
Wiedemann Gertrud,
Hermsen Corinna,
Melzer Michael,
Büttner-Mainik Annette,
Rennenberg Heinz,
Reski Ralf,
Kopriva Stanislav
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.03.034
Subject(s) - physcomitrella patens , sulfite reductase , biology , moss , gene , mutant , gene isoform , reductase , genetics , biochemistry , botany , enzyme
A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens . To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by homologous recombination and subsequently analysed the Δ SiR1 mutants. While Δ SiR1 mutants showed no obvious alteration in sulfur metabolism, their regeneration from protoplasts and their ability to produce mature spores was significantly affected, highlighting an unexpected link between moss sulfate assimilation and development, that is yet to be characterized.