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UV‐induced downregulation of the CDC25B protein in human cells
Author(s) -
Lemaire Matthieu,
Ducommun Bernard,
Nebreda Angel R.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.043
Subject(s) - microbiology and biotechnology , cdc25 , g2 m dna damage checkpoint , downregulation and upregulation , chek1 , phosphorylation , dna damage , mitosis , p38 mitogen activated protein kinases , cyclin dependent kinase 1 , chemistry , cyclin , cell cycle checkpoint , biology , cell cycle , mapk/erk pathway , apoptosis , dna , biochemistry , gene
The CDC25B phosphatase regulates the activation of CDK1–Cyclin B at the onset of mitosis, being a key target of the checkpoint pathways activated by cellular stress and DNA damage. Previous work has reported that checkpoint activation induces the sequestration of CDC25B in the cytoplasm. Here we show that in response to UV irradiation, the levels of CDC25B protein can be downregulated independently of classical checkpoints pathways such as p53, ATM/ATR and p38 MAPK. We also show that translational repression mediated by eIF2α phosphorylation regulates CDC25B expression levels. Taken together, our results illustrate a new mechanism of CDC25B regulation in response to stress.