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NSF independent fusion of Salmonella ‐containing late phagosomes with early endosomes
Author(s) -
Parashuraman Seetharaman,
Madan Richa,
Mukhopadhyay Amitabha
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.040
Subject(s) - phagosome , endosome , vacuole , transferrin receptor , microbiology and biotechnology , lipid bilayer fusion , salmonella , chemistry , transferrin , fusion , biology , receptor , biochemistry , phagocytosis , bacteria , membrane , cytoplasm , genetics , linguistics , philosophy
Initial characterizations of live‐ Salmonella ‐containing early (LSEP) and late phagosomes (LSLP) in macrophages show that both phagosomes retain Rab5 and EEA1. In addition, LSEP specifically contain transferrin receptor whereas LSLP possess relatively more rabaptin‐5. In contrast to LSLP, late‐ Salmonella ‐containing vacuoles in epithelial cells show significantly reduced levels of Rab5 and EEA1. Subsequent results demonstrate that both phagosomes efficiently fuse with early endosomes (EE). In contrast to LSEP, fusion between LSLP and EE is insensitive to ATPγS treatment. Furthermore, LSLP fuses with EE in absence of NEM‐sensitive fusion factor (NSF) as well as in the presence of NSF:D1EQ mutant demonstrating that LSLP fusion with EE is NSF independent.