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GABAA receptor subunit β1 is involved in the formation of protease‐resistant prion protein in prion‐infected neuroblastoma cells
Author(s) -
Kimura Tomohiro,
Ishikawa Kensuke,
Sakasegawa Yuji,
Teruya Kenta,
Sata Tetsutaro,
Schätzl Hermann,
Doh-ura Katsumi
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.029
Subject(s) - gabaa receptor , protein subunit , receptor , gene silencing , biology , microbiology and biotechnology , chemistry , gene , biochemistry
γ‐Aminobutyric acid type A (GABAA) receptor β1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease‐resistant prion protein in prion‐infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decreased the abnormal prion protein level but paradoxically increased the normal prion protein level. Treatment with a gabrb1‐specific inhibitor, salicylidene salicylhydrazide, dose‐dependently decreased the abnormal prion protein level, but silencing of other GABAA receptor subunits’ gene expression and treatments with the receptor antagonists and agonists did not. Therefore, gabrb1 involvement in abnormal prion protein formation is independent of GABAA receptors.