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Recognition of cellooligosaccharides by a family 28 carbohydrate‐binding module
Author(s) -
Tsukimoto Kazuhide,
Takada Rie,
Araki Yuko,
Suzuki Kentaro,
Karita Shuichi,
Wakagi Takayoshi,
Shoun Hirofumi,
Watanabe Takashi,
Fushinobu Shinya
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.027
Subject(s) - cellobiose , cellulase , stacking , chemistry , hydrogen bond , carbohydrate binding module , stereochemistry , crystal structure , ligand (biochemistry) , crystallography , carbohydrate , cellulose , biochemistry , molecule , organic chemistry , receptor
The crystal structures of a carbohydrate‐binding module (CBM) family 28 domain of endoglucanase Cel5A from Clostridium josui have been determined in ligand‐free and complex forms with cellobiose, cellotetraose, and cellopentaose as the first complex structures of this family. In the cleft of a β‐sandwich fold, the ligands are recognized by stacking interactions and hydrogen bonds. Conformations of the bound cellooligosaccharides are similar to those in crystals and solution but clearly different from the cellulose structure. Interestingly, the glucan chain bound on CBM28 is in the opposite direction of that bound to CBM17, although these families share significant structural similarity.