Premium
Generating artificial homologous proteins according to the representative family character in molecular mechanics properties – an attempt in validating an underlying rule of protein evolution
Author(s) -
Liu Xin,
Zhao Ya-Pu
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.010
Subject(s) - character (mathematics) , molecular mechanics , homologous chromosome , protein superfamily , chemistry , computational biology , biology , molecular dynamics , biochemistry , computational chemistry , mathematics , gene , geometry
The molecular mechanics property is the foundation of many characters of proteins. Based on intramolecular hydrophobic force network, the representative family character underlying a protein's mechanics property is described by a simple two‐letter scheme. The tendency of a sequence to become a member of a protein family is scored according to this mathematical representation. Remote homologs of the WW‐domain family could be easily designed using such a mechanistic signature of protein homology. Experimental validation showed that nearly all artificial homologs have the representative folding and bioactivity of their assigned family. Since the molecular mechanics property is the only consideration in this study, the results indicate its possible role in the generation of new members of a protein family during evolution.