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Oxysterol represses high‐affinity IgE receptor‐stimulated mast cell activation in Liver X receptor‐dependent and ‐independent manners
Author(s) -
Nunomura Satoshi,
Endo Kaori,
Makishima Makoto,
Ra Chisei
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.02.006
Subject(s) - degranulation , liver x receptor , oxysterol , immunoglobulin e , receptor , chemistry , microbiology and biotechnology , biology , immunology , nuclear receptor , biochemistry , gene , transcription factor , antibody , cholesterol
Oxysterols activating liver X receptors (LXRs) repress expression of pro‐inflammatory genes and have anti‐inflammatory effects. Here, we show for the first time that bone marrow‐derived murine mast cells (BMMCs) predominantly express LXRβ. 25‐hydroxycholesterol, a representative LXR activating oxysterol, suppressed IL‐6 production and degranulation response in BMMCs following engagement of high‐affinity IgE receptor (FcεRI). Interestingly, 25‐hydroxycholesterol reduced cell‐surface FcεRI expression by inhibiting assembly of FcεRIα and FcεRIβ. We demonstrate that LXR activation was involved in the suppression of IL‐6 production in BMMCs, but that reduced FcεRI expression and degranulation response was mediated in an LXR‐independent manner.