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Involvement of protein kinase Cδ in induction of apoptosis by cationic liposomes in macrophage‐like RAW264.7 cells
Author(s) -
Arisaka Masaya,
Nakamura Tomoko,
Yamada Akiko,
Negishi Yoichi,
Aramaki Yukihiko
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.01.055
Subject(s) - protein kinase c , liposome , cationic liposome , apoptosis , rottlerin , microbiology and biotechnology , reactive oxygen species , chemistry , kinase , biochemistry , biology , transfection , gene
We have recently demonstrated that reactive oxygen species (ROS) play an important role in RAW264.7 cell apoptosis induced by cationic liposomes composed of stearylamine (SA‐liposomes). In this study, we investigated whether protein kinase Cδ PKCδ) is involved in apoptosis induced by cationic liposomes. Tyrosine phosphorylation, nuclear localization, and cleavage of PKCδ were observed following the treatment of cells with SA‐liposomes, suggesting that SA‐liposomes activate PKCδ. Rottlerin, a specific inhibitor of PKCδ, inhibited ROS generation and also suppressed apoptosis. Cell surface proteoglycans may contribute to PKCδ activation by SA‐liposomes. These findings suggest that PKCδ is strongly associated with apoptosis induced by SA‐liposomes.