Premium
MiRNA‐26b regulates the expression of cyclooxygenase‐2 in desferrioxamine‐treated CNE cells
Author(s) -
Ji Yanhong,
He Yonghong,
Liu Le,
Zhong Xingyuan
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.01.036
Subject(s) - gene knockdown , luciferase , nasopharyngeal carcinoma , microrna , cancer research , chemistry , reporter gene , cell culture , cell growth , microbiology and biotechnology , transfection , gene expression , gene , biology , medicine , biochemistry , genetics , radiation therapy
Here we report that miR‐26b is involved in COX‐2 overexpression in desferrioxamine (DFOM)‐treated carcinoma of nasopharyngeal epithelial (CNE) cells. The level of miR‐26b in DFOM‐treated CNE cells is inversely proportional to the expression level of the COX‐2 protein. Overexpression of miR‐26b in DFOM‐treated CNE cells inhibits cell proliferation. A luciferase reporter gene experiment suggests that the 3′ untranslated region of COX‐2 carries a binding site for miR‐26b. Overexpression of miR‐26b marginally reduces the levels of COX‐2 protein in DFOM‐treated CNE cells. Moreover, knockdown of COX‐2 expression had a similar effect to overexpression of miR‐26b. Taken together, these results suggest that miR‐26b regulates COX‐2 expression in DFOM‐treated cells.