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The C‐terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA‐binding
Author(s) -
Sueda Kae,
Shimura Hanako,
Meguro Ayano,
Uchida Takeshi,
Inaba Jun-ichi,
Masuta Chikara
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.01.033
Subject(s) - escherichia coli , cucumber mosaic virus , terminal (telecommunication) , dna , chemistry , biology , microbiology and biotechnology , virus , biochemistry , virology , plant virus , gene , computer science , telecommunications
The RNA silencing suppressor 2b protein of Cucumber mosaic virus (CMV) is difficult to produce in Escherichia coli . We compared two CMV 2b proteins that differ in their toxicity against E. coli and found that the acidic amino acid residues in the C‐terminal significantly affected the toxicity and expression level of the protein in E. coli . In addition, in a DNA‐binding assay, 2b had the ability to bind to DNA, and this ability was affected by the charge on the C‐terminal residues of 2b. We concluded that the C‐terminal residues were important for 2b's DNA‐binding ability, which may partly explain the toxicity of the protein.