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Influence of the hepatitis C virus 3′‐untranslated region on IRES‐dependent and cap‐dependent translation initiation
Author(s) -
Bung C.,
Bochkaeva Z.,
Terenin I.,
Zinovkin R.,
Shatsky I.N.,
Niepmann M.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.01.015
Subject(s) - internal ribosome entry site , five prime untranslated region , untranslated region , translation (biology) , virology , hepatitis c virus , three prime untranslated region , eukaryotic translation , biology , messenger rna , virus , microbiology and biotechnology , genetics , gene
Translation of hepatitis C virus (HCV) genomic RNA is directed by an internal ribosome entry site (IRES) in the 5′‐untranslated region (5′‐UTR), and the HCV 3′‐UTR enhances IRES activity. Since the HCV 3′‐UTR has a unique structure among 3′‐UTRs, we checked possible communication between the 5′‐ and the 3′‐UTR of HCV during translation using chimeric reporter RNAs. We show that translation directed by the HCV IRES and by the HCV‐like IRES of porcine teschovirus (PTV) which belongs to a quite distinct family of viruses (picornaviruses) or by the EMCV IRES is also enhanced by the HCV 3′‐UTR or by a poly(A)‐tail in different cell types.