Premium
Use of a redox‐sensing GFP (c‐roGFP1) for real‐time monitoring of cytosol redox status in Arabidopsis thaliana water‐stressed plants
Author(s) -
Jubany-Mari T.,
Alegre-Batlle L.,
Jiang K.,
Feldman L.J.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2010.01.014
Subject(s) - redox , cytosol , green fluorescent protein , arabidopsis , chemistry , water stress , biophysics , arabidopsis thaliana , biochemistry , biology , botany , enzyme , inorganic chemistry , mutant , gene
Using Arabidopsis plants transformed with a redox‐sensing green fluorescent protein targeted to the cytosol (c‐roGFP1), we have demonstrated, in real time, measurements of reversible changes of redox status in the cytosol of plants subjected to a gradual water‐stress, followed by re‐watering. Plants sensed water stress, and changed the redox potential of their cytosol to a more oxidized value after a gradually‐imposed water stress. Small variations in the cytosol redox potential and ascorbate (AA) values suggest that this parameter was tightly regulated. The re‐watering was paralleled by a return of water stress, redox potential and ascorbate to initial values, showing the reversibility of water stress and its consequences.